The COVID-19 RT-PCR Test is for the qualitative detection of nucleic acid from SARS-CoV-2 in upper and lower respiratory
specimens collected from individuals suspected of COVID-19, as well as upper respiratory specimens collected from any
individual, including individuals without symptoms.
Polymerase chain reaction (PCR) is a relatively simple and widely used molecular biology technique to amplify and detect DNA
and RNA sequences. Unlike the conventional methods of DNA cloning and amplification, which can often take days, instead PCR
requires only a few hours. PCR is highly sensitive and requires minimal template for detection and amplification of specific
sequences. Reverse transcription PCR, or RT-PCR, allows the use of RNA as a template. An additional step allows the detection
and amplification of RNA.
This tests for the presence of the actual virus’s genetic material or its fragments as it breaks down. This is the most reliable and
accurate test for detecting active infection. The main advantages of COVID-19 PCR test are its accuracy and reliability.